Friday, 13 November 2015

Effects of increased temperature on DMS(P) concentration in coral associated Symbiodinium

Worldwide coral reefs face climate change. Recent events have shown that changes in coral reef ecosystems can lead to disease such as bleaching. Anyhow, coral reefs have existed for hundreds of thousands of years and have survived climate change events before. To understand how coral reefs respond or even adapt to climate change, it is necessary to have a closer look on the small things in life of a coral.
Most reef building corals are constructed trough the association between coral polyps and their symbiotic dinoflagellate microalgae. Besides providing the host with fixed carbon, the zooxanthellae (genus Symbiodinium) also contribute to the production of antioxidants to prevent oxidative damage. As part of the coral holobiont zooxanthellae produce and process dimethylsulphoniopropionate (DMSP) and its enzymatic cleavage product dimethylsulphide (DMS).
DMSP and DMS play a key role in the global sulphur cycle and also in the antioxidant system of the coral.
Depending on their symbiotic zooxanthaellae corals respond differently to climate change. Some clades of zooxanthallea are known to be more resistant to e.g. temperature changes. Increasing temperature is one of the main factors for coral bleaching.

The study by Deschaseeaux et al. (2014) investigated the tolerance of the symbiotic zooxanthellae to increasing temperature linked to the production of DMSP and DMS. Therefore, two different subclades of symbiotic algae (Acropora millepora (D1) and Acropora tenuis (C1)) were collected in waters of Magnetic Island, Australia. Clade D is known to be more resistant to thermal stress than clade C.
The two clades were isolated from the coral tissue. To prevent contamination the zooxanthellae were kept in an axenic chamber. The genotypes of the clades were identified. Clades were examined in chambers with increasing temperature and in control chambers. DMSP and dissolved and gaseous DMS were measured.

Relevant results were that DMSP and DMS concentration differed between the two clades. DMSP concentration decreased in all treatments. Although, concentration of DMSP were higher in clade C than in D. No difference in DMSP concentration was measured between the two treatments of clade D. For clade C the concentration of DMSP was higher in the control chamber than in the chamber with increased temperature. The higher concentration of DMSP in the control chamber might be a result of the enhanced DMSP consumption in clade C with increasing temperature. Suggesting that clade C uses DMSP to prevent oxidative stress. This is supported by the results of measured DMS. Decreasing concentration of DMSP didn’t lead to increasing concentration of gaseous DMS in clade C. Potentially caused by consumption of DMS under thermal stress. All in all, clade C showed distinct response to thermal stress, whereas clade D remained nearly unaffected.

Despite of some vague results, this study contributes to a better understanding of coral reef response to increasing temperature. Considering the theory that coral produced DMS plays a role in cloud forming and therefore has an impact on local climate, enhanced consumption of DMS(P) due to thermal stress by clade C could affect this interaction. Secondly, it can be assumed that increasing temperature has distinct effects on the biogenic sulphur cycle of symbiotic zooxanthellae.
Furthermore, this study focused only on the DMS(P) production by symbiotic zooxanthellae. The whole microbial holobiont should be considered for further studies.

Additionally, I would like to recommend this paper by Nils Rรคdecker, a master student of the Centre for Tropical Marine Ecology, Bremen. The paper gives you a nice overview of nitrogen cycling in corals. Even with a short video at the end: http://www.sciencedirect.com/science/article/pii/S0966842X1500075X

E.S.M. Deschaseaux, V.H. Beltran, G.B. Jones, M. A. Deseo, H.B. Swan, P.L. Harrison, B.D. Eyre (2014) Comparative response of DMS and DMSP concentrations in Symbiodinium clades C1 and D1 under thermal stress. Doi: 10.1016/j.jembe.2014.05.018. http://www.sciencedirect.com/science/article/pii/S0022098114001373

1 comment:

  1. Since I have read the paper "A bacterial pathogen uses DMSP as a cue to target heat-stressed corals" (Garren et al. 2014) which Katherine and I presented in the seminar, I see issues in this paper. Based on their results Deschaseeaux et al. (2014) conclude that thermal stress leads to decreasing DMSP concentration and reduced DMS emission which would have a negative feedback on the DMS-CCN theory. Though, they have only measured the DMS(P) concentration of the associated zooxanthellae. In my opinion, it is necessary to consider always the entire coral holobiont to make those predictions.
    In the paper by Garren et al. (2014) they mention a fivefold increase of DMSP in the coral mucus under thermal stress. Even though they used a different coral species (still a stony coral from a reef close by) and applied a different temperature treatment, they considered the mucus from the entire coral holobiont.

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